EFFECT OF LIQUID YEAST CULTURE WITH PLANT HERBAL...
EFFECT OF LIQUID YEAST CULTURE WITH PLANT HERBAL EXTRACTS ON
SOME CARCASS CHARACTERISTICS OF SWINE




RICMAR D. CALABIAS




A THESIS MANUSCRIPT SUBMITTED TO THE COLLEGE OF AGRICULTURE
BENGUET STATE UNIVERSITY, IN PARTIAL FULFILLMENT
OF THE REQUIREMENTS
FOR THE DEGREE








BACHELOR OF SCIENCE IN AGRICULTURE
(ANIMAL SCIENCE)








APRIL, 2012
Effect Of Liquid Yeast Culture With Plant Herbal Extracts On Some Carcass
Characteristics Of Swine
RICMAR D. CALABIAS. APRIL 2012

INTRODUCTION
The gross structure of meat consists of lean, fat, and bone. Among the three
components, lean is considered as the most important since it comprises the bulk of carcass,
50-65% of the carcass weight. Fat is the most variable component of meat. It comprises 5-
30% of carcass. Older animals tend to contain higher amount of fat. Bone, on the other
hand, comprises 15-17% of carcass.
Feed additives are compounds added to swine diets for the purpose of enhancing
animal performance, either directly or indirectly. These compounds may elicit a response,
and that response of the pig’s energy, amino acid, and vitamin/mineral requirements.
However, the response is dependent on age of pig, disease level, genetics, environment
factors, and type of diet/feedstuffs. The purpose of this paper is to provide an overview of
the feed additives currently used in swine production, and to provide background
information in order to optimize their use.
One of the products that can be used as feed additives is a liquid yeast culture with
herbal extracts. This feed additive is a liquid yeast culture rich in fermentation metabolites
and herbal extracts composed of organic acids and organic minerals. According to the
manufacturer, this feed additive function is to protect the animal’s digestive system from
the harmful effects of toxins, parasites, and bacterial pathogens whether they are in water
or in feeds. Its unique combination of yeast/phototrophic culture and herbal extracts keeps
the digestive system healthy for optimum absorption of feed nutrients. Ammonia emissions
are dramatically reduced resulting in minimal upper respiratory challenges thus lowering
antibiotics cost.
Effect Of Liquid Yeast Culture With Plant Herbal Extracts On Some Carcass
Characteristics Of Swine
RICMAR D. CALABIAS. APRIL 2012

The manufacturer’s claimed characteristics of the liquid yeast culture with plant
herbal extracts as feed additives that makes the product economically viable when used in
feeds or in water are as: probiotic, prebiotic, acidifier, metabolic activity enhancer,
ammonia odor suppressant, immunity enhancer as an anti-parasitic agent. This feed
additive has been used successfully in broiler, swine, cattle fattening and layer farms.

Information generated from this study maybe used by swine raisers, students, meat
processors, and consumers. It may also serve as reference for students and other researchers
in coming up with other related studies
Generally, this study was conducted to determine the effect of liquid yeast culture
with plant herbal extract on some carcass characteristics of swine.
Specially, it aimed to determine the effect of liquid yeast culture with plant herbal
extracts on back fat thickness, dressing percentage, percent abdominal fat, percent whole
sale cuts and edible entrails, loin eye, and carcass length of swine.



This study was conducted at Banig, Tawang, La Trinidad, Benguet from February
to March, 2012.







Effect Of Liquid Yeast Culture With Plant Herbal Extracts On Some Carcass
Characteristics Of Swine
RICMAR D. CALABIAS. APRIL 2012



REVIEW OF LITERATURE
Analyses of liquid yeast culture with plant herbal extracts as recommended by
Gallali IZW Farm Products (2010) are the following:


Yeast



370.000 per gram


Phototrophic


13.000 per gram


pH




4.0-5.0


Ca
422 Mg/L

P
100 Mg/L


K
429 Mg/L

Mg
149 Mg/L

Fe
41 Mg/L

Mn
1.4 Mg/L


Se
0.0002 Mg/L
Others-traces


Cu
0.07 Mg/L
Probiotics
Extensive investigation and research has been carried out on those probiotics
approved. For used and their benefits as stated by Sainsbury (1998) may be summarized as
follows: (a) probiotics can promote growth and productivity in a natural way; (b) it may
protect against salmonella infections; (c) they can protect against toxins produced by
harmful forms of E. Coli; (d) it stimulate immunity to infections by boosting interferon
production, immunoglobin concentration and macrophage activity; (e) probiotic suppress
clostridial infestion which are often associated with intensive livestock production.

Probiotics are beneficial microorganism (mainly lactic acid producing bacteria and
yeast combinations) added to rations to improve the intestinal microbial balance of the
animal (PCCARD, 2005).These bacteria exert their beneficial effects through “competitive
exclusion and lactic acid production. In addition to competitive exclusion is reduction in
Effect Of Liquid Yeast Culture With Plant Herbal Extracts On Some Carcass
Characteristics Of Swine
RICMAR D. CALABIAS. APRIL 2012

gut pH, which makes the intestinal environment unfavourable for certain pathogenic
bacteria. There has also been increasing evidence that an acidic environment is conductive
to increased enzymatic activity within the digestive system that leads to improved
production performance (PCCARD, 2002).

Over the last 10-15 years, probiotics have been proposed as an alternative to
antibiotics (Jensen, 2010). On the other hand, Close and Alberta (2010) said that unlike
antibiotics, probiotics introduce live beneficial bacteria into the intestinal tract.
Accordingly, several authors have assessed the efficacy of probiotics as growth promoters
for pigs. Most concluded that when result is averaged over several trials, there is an
improvement in growth rate and in the efficiency of feed utilization. However, the results
are highly variable. The effects of the may be probiotics more consistent and positive in
piglets than in growing-finishing animals.

Prebiotics
Common examples of prebiotics used in research or on farms include
oligosaccharides (McDonald et al., 2002), oligofructose, fructooligosaccharide (FOS),
mannanoligosaccharides (MOS), dietary fibers and insulin. Oligosaccharides have been
claimed as beneficial nutritional modifiers for monogastric farm animals. They fall into the
group of materials also known as prebiotics, which are defined as compounds, other than
dietary nutrients, that modify the balance of the microbial population by promoting the
growth of beneficial and thereby provide a healthier.




Certain dietary fibers have been shown to improve intestinal secretions and growth
of the digestive mucosa (Mateos et al., 2000) and a number of different fiber fractions have
been tested for their ability to enhance pig growth and suppress pathogenic bacteria
Effect Of Liquid Yeast Culture With Plant Herbal Extracts On Some Carcass
Characteristics Of Swine
RICMAR D. CALABIAS. APRIL 2012

colonization. The mode of action of the dietary fibers is believed to depend on the specific
fraction in question readily fermentable non-digestible oligosaccharides (e.g. fructo-
oligosaccharide, galacto-oligosaccharide, and Transco-galactosaccharides) are believed to
improve pig performance by stimulating the proliferation of Bifidobacteria in the large
intestine, which in turn reduces colonic pH and increases the concentration of lactic acid
(Houdijk et al., 2002).

Acidifier


Acidifier is made of organic acid or mixture of organic acid when incorporated in
the ration. They exert their effect on the gastrointestinal environment by lowering the
stomach pH. Low stomach pH prevents the growth band proliferation of pathogenic
microorganisms, thus promoting better feed efficiency (PCCARD, 2000).

Acidifier are believed to enhance growth by improving gut health through reduction
of pH and buffering capacity of diets, improvement of pancreatic sections that increase
nutrient digestibility, or promotion of beneficial bacterial growth while inhibiting growth
of pathogenic microbes (Jacela et al., 2009).

Acids generally lowered the pH and buffering capacity of the diet, reduced the pH
within the stomach, increase nutrients digestible, promote the performance and improved
feed efficiency. The growth promoting effects of acids are most prominent in the first few
weeks after weaning (Close and Alberta, 2010).



Effect Of Liquid Yeast Culture With Plant Herbal Extracts On Some Carcass
Characteristics Of Swine
RICMAR D. CALABIAS. APRIL 2012



MATERIALS AND METHODS


Materials
The materials used were six heads of swine given yeast culture with plant herbal
extract as feed additive and commercial ration.

The other materials that were used include the following: livestock scale, measuring
tape, weighing scale, knives, bolo, stunner, LPG, blow torch, lighter, chopping board,
water, and basin.
Preparation of the Ration
The yeast culture was mixed thoroughly with the commercial ration following the
dosage recommended by the manufacturer. The feed mixture was given immediately to the
pigs after mixing. The dosages of the feed yeast culture to be mixed with the commercial
ration were as follows:

Weaners



1.5ml per kilogram of feeds

Starters



0.63ml per kilogram of feeds

Growers



0.40ml per kilogram of feeds

Finishers



0.88ml per kilogram of feeds
Experimental Treatments

The ten weaned pigs were grouped into two treatments following the completely
randomized design (CRD). Each treatment was replicated five times with one pig per
replication. The individual weights of the experimental animals was taken first and
recorded before placing them into their respective pens. The following were the different
treatments in the study.
Effect Of Liquid Yeast Culture With Plant Herbal Extracts On Some Carcass
Characteristics Of Swine
RICMAR D. CALABIAS. APRIL 2012

T0- without liquid yeast culture with plant herbal extracts additive

T1- with liquid yeast culture with plant herbal extract additive
All experimental animals in each factor were subjected to uniform slaughtering
procedures. The following approved practices in slaughtering hogs were followed:
1. Stunning. This is the process of making animals unconscious, prior to bleeding. It is
using a GI pipe applied on the forehead at the cross section of the imaginary line between
the eye and ears (Figure 1).
2. Sticking. It is bleeding the animal with the used of seven-inch sticking knife. The
head is held with the left hand and sticking is done on the hallow portion above the tip of
the breast bone (Figure 2).
3. Singeing. Application of flame to a carcass for the purpose of burning the uncraped
or unshaved hair and killing some microorganism (Figure 3).


Figure 1. Stunning the Hog
Effect Of Liquid Yeast Culture With Plant Herbal Extracts On Some Carcass
Characteristics Of Swine
RICMAR D. CALABIAS. APRIL 2012

4. Evisceration. It involves the opening of the carcass up to the complete removal of
the internal organ from the body cavity (Figure 4).
5. Weighing the Carcass. It is the process of weighing the hog after slaughter excluding
the entrails, head and feet (Figure 5).
6. Fabrication. Cutting the carcass into whole sale cuts, namely: shoulder, ham, belly,
and loin, then weighing ( Figure 6 to 9).


Figure 2. Sticking the Hog
Effect Of Liquid Yeast Culture With Plant Herbal Extracts On Some Carcass
Characteristics Of Swine
RICMAR D. CALABIAS. APRIL 2012

Figure 3. Singeing and Scalding the Hog


Figure 4. Evisceration of Hog
Effect Of Liquid Yeast Culture With Plant Herbal Extracts On Some Carcass
Characteristics Of Swine
RICMAR D. CALABIAS. APRIL 2012

Figure 5. Weighing the Carcass Hog


Figure 6. Weighing the Ham
Effect Of Liquid Yeast Culture With Plant Herbal Extracts On Some Carcass
Characteristics Of Swine
RICMAR D. CALABIAS. APRIL 2012

Figure 7. Weighing the Shoulder


Figure 8. Weighing the Belly
Effect Of Liquid Yeast Culture With Plant Herbal Extracts On Some Carcass
Characteristics Of Swine
RICMAR D. CALABIAS. APRIL 2012


Figure 9. Weighing the Loin
Data to be Gathered
1. Slaughter and Carcass Data
a. Slaughter weight (kg). This was obtained by weighing the animals prior
to slaughter using livestock scale after 24 hours of fasting.

b. Carcass weight (kg). It is the weight of the carcass without head, feet and
the entrails.
c. Weight of wholesale cut (kg). The carcass was chopped into wholesale
cuts which were being weighed individually with a meat scale.
d. Weight of edible entrails (kg). Edible entrails were separated from the
carcass and then weighed. Edible entrails consisted of heart, liver, lungs, spleen,
kidneys, pancreas and intestines.
Effect Of Liquid Yeast Culture With Plant Herbal Extracts On Some Carcass
Characteristics Of Swine
RICMAR D. CALABIAS. APRIL 2012

2. Carcass Measurement
a. Carcass length (cm). This was taken by measuring from the first rib to the
base of the tail on the suspended carcass in centimetre (Figure 10).
b. Back fat measurement (cm). This was measured from the first rib (P1),
last rib (P2), and at the lumbar vertebra (P3) of the splitted carcass (Figure 11).
c. Loin eye area (cm2). This was taken by cutting the loin between the tenth
and eleventh rib; the muscle area was carefully traced on an ordinary graphing paper. An
ordinary graphing paper was placed at the bottom of the tracing on the acetate paper and
the number of squares will be counted. Meat graphing papers are calibrated twenty squares
per square inch. The area was been determined using an ordinary graphing paper. The
formula that was used was length x width x 0.8. Length was the longest straight line that
can be made within the tracings while the width will be the straight line that bisects the
length at right angle (Figure 12).


From the above data, the fallowing parameters were computed:
1. Carcass yield. The carcass yield was expressed in terms of dressing
percentage using the formula:
Dressing Percentage= Carcass Weight x 100







Slaughter Weight


2. Percentage of abdominal fat


Percent of Abdominal Fat =Weight of Abdominal Fat x 100
Carcass weight
3. Percentage of wholesale cuts


Percent of Wholesale Cuts = Cut weight x 100






Carcass weight
4. Percent of edible entrails
Effect Of Liquid Yeast Culture With Plant Herbal Extracts On Some Carcass
Characteristics Of Swine
RICMAR D. CALABIAS. APRIL 2012



Percent of Edible Entrails =Weight of edible entrails x 100






Slaughter weight
5. Back fat thickness. This was determined by computing the average of
back fat measurement taken at the opposite of P1 (first rib), P2 (last rib) and P3 (lumbar
vertebra).

Figure 10. Measuring the carcass length




Effect Of Liquid Yeast Culture With Plant Herbal Extracts On Some Carcass
Characteristics Of Swine
RICMAR D. CALABIAS. APRIL 2012


Figure 11. Measuring the back fat thickness


Figure 12. Measuring the loin eye area

Effect Of Liquid Yeast Culture With Plant Herbal Extracts On Some Carcass
Characteristics Of Swine
RICMAR D. CALABIAS. APRIL 2012

RESULT AND DISCUSSION
Slaughter Weight, Carcass Weight
and Dressing Percentage of Hogs
Table 1 shows the slaughter weight and the dressing percentage of the slaughtered
hogs. Statistical analysis showed no significant differences in the slaughter weight, carcass
weight, and dressing percentage of hogs feed with pure commercial feeds and those given
with pure commercial feeds with liquid yeast culture with plant herbal extracts.
Dressing percentage of slaughtered animals is an indicator of carcass yield. The
average dressing percentage of hogs used in this study was 71.54 % for hogs weighing
about 85.83 kg slaughter weight. This is relatively higher than the dressing percentage of
69.93% reported by Ibarra (1983) from hogs with a slaughter weight of 87.80 kg. The small
discrepancy may be due to the condition of the carcass when the carcass weights were
taken. In this study, carcass weight was taken from a freshly slaughtered hog while that in
Ibarra (1983), it was taken from chilled carcass. It has been reported that carcass weight
tends to decrease by 2% after it has been chilled (Ibarra, 1983).

Table 1. Effect LYC with PHE on the slaughter weight, carcass weight and dressing
percentage of hogs













TREATMENTS SLAUGHTER CARCASS DRESSING
WEIGHT WEIGHT PERCENTAGE
(Kg) (Kg) (%)













Without LYC with PHE 85.33a 61.50a 72.07a
With LYC with PHE 84.33a 61.32a 71.01a













*LYC with PHE- liquid yeast culture with plant herbal extract

Percent Weight of Cuts
Effect Of Liquid Yeast Culture With Plant Herbal Extracts On Some Carcass
Characteristics Of Swine
RICMAR D. CALABIAS. APRIL 2012

Table 2 shows the weight of shoulder, ham, belly and loin expressed as percent of
slaughter weight of hogs with an average slaughter weight of 85.83 kg. Statistical analysis
showed no significant differences in the percent shoulder, ham and loin of hogs given
commercial feeds and those given with pure commercial feeds with liquid yeast
culture(LYC) with plant herbal extract. The average percent of shoulder, ham and loin of
85.83 kg hogs were 20.39%, 21.59% and 14.16%, respectively. On the other hand,
statistical analysis showed a highly significant difference in the percent belly between
treatments. The percent belly of hogs given no LYC supplement (16.31%) was higher thnt
the percent belly of hog given LYC (13.40%). The lower percent belly in hogs given LYC
could be attributed to a lesser layer of fat observed in this cut.
Compared to the standard average yield in hogs stated by Ibarra (1983), the percent
whole sale cuts obtained in this study is relatively higher. The percent whole sale cuts of
87.90 kg reported by Ibarra were 15.99% shoulder, 16.74% loin, 19.20% ham, 16.37%
belly having a slaughter weight of 87.90 kg.

Table 2. Whole sale cuts of swine expressed as percent of slaughter weight













TREATMENTS PERCENT PERCENT PERCENT PERCENT
SHOULDER HAM LOIN BELLY














Without LYC with PHE 20.39a 21.59a 14.16a 16.31a
With LYC with PHE 20.14a 22.10a 14.90a 13.40b













*LYC with PHE- liquid yeast culture with plant herbal extract







Effect Of Liquid Yeast Culture With Plant Herbal Extracts On Some Carcass
Characteristics Of Swine
RICMAR D. CALABIAS. APRIL 2012

Table 3. Whole sale cuts of swine expressed as percent of carcass weight













TREATMENTS PERCENT PERCENT PERCENT PERCENT
SHOULDER HAM LOIN BELLY













Without LYC with PHE 28.34a 29.95a 19.66a 22.64a
With LYC with PHE 28.71a 31.12a 21.00a 18.87b













*LYC with PHE- liquid yeast culture with plant herbal extract
Table 3 shows the weight of shoulder, ham, belly and loin expressed as percent of
carcass weight of hogs with an average slaughter weight of 85.83 kg. Statistical analysis
showed no significant differences in the percent shoulder, ham, and loin of hogs between
treatments. The average percent of shoulder, ham and loin of 85.83 kg hogs were 28.34%,
29.95% and 19.66%, respectively. On the other hand, statistical analysis showed a highly
significant different in the percent of belly. The percent belly of hogs given no LYC
supplement (22.64%) was higher than the percent belly of hog given LYC (18.87%).
The trend is similar when the weights of cuts were expressed as percent of slaughter
weight and as percent of carcass weight.

Percent of Abdominal Fat
and Percent of Edible
Entrails of Hogs
Table 4 shows the weight of abdominal fat and edible entrails of hogs expressed as
percent of carcass weight. Statistical analysis showed a highly significant difference in
percent abdominal fat between treatments. The percent abdominal fat of hogs given no



Effect Of Liquid Yeast Culture With Plant Herbal Extracts On Some Carcass
Characteristics Of Swine
RICMAR D. CALABIAS. APRIL 2012

Table 4. Effect of liquid yeast culture with plant herbal extract on the abdominal fat and
edible entrails of hogs













TREATMENTS PERCENT PERCENT
ABDOMINAL FAT EDIBLE ENTRAILS














Without LYC with PHE 1.93b 13.10a
With LYC with PHE 2.93a 12.84a













*LYC with PHE- liquid yeast culture with plant herbal extract
LYC supplement (1.93%) was lower than the percent belly of hog given LYC
(2.93%). It appears that more fat was deposited in the abdominal with LYC
supplementation.
Statistical analysis showed no significant differences in percent edible entrails
between treatments. Edible entrails included lungs, liver, heart, spleen, stomach and small
intestines. The average percent edible entrails in 85.83 kg hogs was 18.13%.

Back Fat Thickness
Table 5 shows the back fat thickness hogs slaughtered at an average weight of 85.83
kg. Back fat measurement was taken at the back of the animal one inch below the midline
in three locations: opposite of first rib (P1), opposite of last rib (P2) and opposite of lumbar
vertebra (P3). Statistical analysis showed no significant differences between treatments in
the backfat thickness measured at P1 and P3. The average back fat measurements of hogs
slaughtered at 85.83 kg taken at P1 and P2 were 3.0cm and 2.17 cm, respectively. However,
the back fat thickness taken at P2 of hogs given no LYC supplement (3.0 cm) was
significantly higher than that taken from hogs given LYC (2.6 cm) supplement.
Effect Of Liquid Yeast Culture With Plant Herbal Extracts On Some Carcass
Characteristics Of Swine
RICMAR D. CALABIAS. APRIL 2012

No significant differences were obtained when the average of the three back fat
measurements was taken, despite a significant difference in the P2 measurement.

Carcass Length and Loin Eye Area
Table 6 present the average carcass length and the loin eye area. Statistical analysis
revealed no significant difference between treatments. The average carcass length was
67.00 cm and the average loin eye area was 28.91 cm for hogs slaughtered at an average
of 85.83 kg. The carcass length was taken at the tip of symphysis pubis to the forward edge
of the first rib. However the loin eye area was computed by multiplying the length and
width by 0.08 cm.

Table 5. Back fat thickness of hogs given diets with or without liquid yeast culture with
plant herbal extract (cm)













TREATMENTS BACK FAT BACK FAT BACK FAT
THICKNESS THICKNESS THICKNESS
(P1,) (P2) ( P3)
(cm) (cm) (cm)














Without LYC with PHE 3.00a 3.00a 2.33a
With LYC with PHE 3.00a 2.60b 2.00a













*LYC with PHE- liquid yeast culture with plant herbal extract


Table 6. Measurement of carcass length and loin eye area













TREATMENTS CARCASS LENGHT LOIN EYE AREA
(cm) (cm)














Without LYC with PHE 66.33a 18.17a
With LYC with PHE 67.67a 18.09a













*LYC with PHE- liquid yeast culture with plant herbal extract
Effect Of Liquid Yeast Culture With Plant Herbal Extracts On Some Carcass
Characteristics Of Swine
RICMAR D. CALABIAS. APRIL 2012


SUMMARY, CONCLUSION AND RECOMMENDATION
Summary
The study was conducted to determine the effect of liquid yeast culture with plant
herbal extract on dressing percentage, whole sale cuts, abdominal fat, edible entrails, back
fat thickness, carcass length and loin eye area. Six hogs were distributed to two treatments:
pure commercial feeds and commercial feeds supplemented with liquid yeast culture
(LYC) with plant herbal extract. Each treatment has three replicates.

Data were analyzed using analysis of variance appropriated for CRD. Duncan’s
Multiple Range Test was used to compare treatment means.

Result showed that supplementing swine rations with liquid yeast culture with plant
herbal extract has no significant effect on the dressing percentage, whole sale cuts like
shoulder, ham, and loin expressed as percent of carcass weight, weight of edible entrails
expressed as percent of slaughter weight, carcass length and average back fat thickness.
The average dressing percentage of approximately 85 kg hogs was 71.54%. The wholesale
cuts expressed as percent of carcass weight were the following; 20.27% shoulder, 21.85%
ham, 14.53% loin. While the average weight of edible entrails express as percent of
slaughter weight was 12.97%. The averages of carcass length and back fat thickness were
67 cm and 2.65 cm.

On the other hand, highly significant differences in the weight of belly expressed
as percent of carcass weight, back fat thickness at P2 and weight of abdominal fat expressed
as percent of slaughter weight. The weight of belly expressed as percent of carcass weight
of hogs given no LYC supplement (22.64%) was significantly higher than that of hogs
given the LYC supplement (18.87%). Similarly, back fat thickness at P2 of hogs given no
Effect Of Liquid Yeast Culture With Plant Herbal Extracts On Some Carcass
Characteristics Of Swine
RICMAR D. CALABIAS. APRIL 2012

LYC supplement (3.00 cm) was significantly higher than that in hogs given the LYC
supplement (2.60 cm). On the other hand, weight of abdominal fat expressed as percent of
carcass weight in hogs given no LYC supplement (1.93 %) was significantly lower than
that in hogs given the LYC supplement (2.93%).

Conclusion

Based on the results of the study, liquid yeast culture with plant herbal extracts may
be used as supplement to swine as appears to lower the fat in the belly and in back fat in
the region of the last rib.

Recommendation
Based on the findings, liquid yeast culture with plant herbal extract may be used as
feed supplement in swine rations.













Effect Of Liquid Yeast Culture With Plant Herbal Extracts On Some Carcass
Characteristics Of Swine
RICMAR D. CALABIAS. APRIL 2012



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Effect Of Liquid Yeast Culture With Plant Herbal Extracts On Some Carcass
Characteristics Of Swine
RICMAR D. CALABIAS. APRIL 2012

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Effect Of Liquid Yeast Culture With Plant Herbal Extracts On Some Carcass
Characteristics Of Swine
RICMAR D. CALABIAS. APRIL 2012