BIBLIOGRAPHY PEG-ED, JIMUEL P. OCTOBER ...
BIBLIOGRAPHY
PEG-ED, JIMUEL P. OCTOBER 2012. Acclimatization of Tissue Cultured
Banana Plantlets (var. ‘Lakatan’) as Affected by Different Growing Media. Benguet State
University, La Trinidad, Benguet.
Adviser: Leila Mary A. Ayban, MSc.
ABSTRACT
The study was conducted at the Ornamental Horticulture Area, Benguet State
University, La Trinidad, Benguet from March 2012 to May 2012. The study was conducted
to establish the best growing media to harden the rooted banana plantlets from in vitro
before transplanting them to the field; and to evaluate the growth of banana plantlets from
in vitro grown in different growing media and media compositions.
Results revealed that a medium of fern dust alone; and alnus compost + burnt rice
hull+ fern dust+ Enrico Organic Growing media significantly promoted more vigorous
plantlets and enhanced better growth and development of banana plantlets. Moreover
plantlets grown in fern dust produced the longest roots and higher number of leaves per
plant.
Furthermore, banana plantlets grown in the different growing media had no
significant differences in terms of percentage survival, which was recorded 45 days after
transplanting from the culture bottles.
In addition, fern dust alone; and alnus compost + burnt rice hull+ fern dust+ Enrico
Organic growing media were found to be the best growing media in acclimatizing banana
plantlets from in vitro.
Acclimatization of Tissue Cultured Banana Plantlets (var. ‘Lakatan’) as Affected by
Different Growing Media | PEG-ED, JIMUEL P. OCTOBER 2012
RESULTS AND DISCUSSIONS
Plant Vigor
Table 1 shows the plant vigor of banana plantlets grown in different growing media
after 45 days. Plantlets grown in sandy soil were significantly more vigorous with a mean
rating of 2.33 but were not significantly different to those plantlets grown in garden soil +
alnus compost (farmers practice), and inalnus compost only, burnt rice hull only, and
Enrico Organic growing media. Moreover, plantlets grown in fern dust only and alnus
compost + sand + burnt rice hull + fern dust + Enrico Organic Growing Media were the
least vigorous.
Table 1. Plant vigor rating index of banana plantlets after 45 days from planting
TREATMENT
MEAN
Farmers practice (garden soil and alnus compost)
2.00a
Alnus Compost
2.00a
Sandy Soil
2.33a
Burnt rice hull
2.00a
Fern dust
1.00b
Enrico Organic Growing Media
2.00a
AC + S + BRH + FD +Enrico Organic Growing Media
1.00b
CV (%)
12.39
%
Means with common letter are not significantly different at 5% level of DMRT
Acclimatization of Tissue Cultured Banana Plantlets (var. ‘Lakatan’) as Affected by
Different Growing Media | PEG-ED, JIMUEL P. OCTOBER 2012
Plant Height
As presented in Table 2 banana plantlets grown in alnus compost + sand + burnt
rice hull + fern dust + Enrico Organic Growing Media had significantly faster plant growth
which was not significantly different with those of the plantlets grown in fern dust onlyas
compared to the other growing media.
The presence of sand and burnt rice hull in the alnus compost media improved the
physical properties of the medium resulting to better drainage and aeration leading to better
plant growth performance (Dumaslan, M. 2006).
Table 2. Plant height of banana plantlets after 45 days from planting
TREATMENT
MEAN
(cm)
Farmers practice (garden soil and alnus compost)
12.56b
Alnus Compost
12.33 b
Sandy Soil
12.60 b
Burnt rice hull
14.03 b
Fern dust
26.33a
Enrico Organic Growing Media
12.57 b
AC + S + BRH + FD +Enrico Organic Growing Media
28.23a
CV (%)
11.65%
Means with common letter are not significantly different at 5% level of DMRT
Acclimatization of Tissue Cultured Banana Plantlets (var. ‘Lakatan’) as Affected by
Different Growing Media | PEG-ED, JIMUEL P. OCTOBER 2012
Root Length
Table 3.Shows that significant or differences were observed among the different
growing media used. Banana plantlets grown in fern dust only with a mean of 30.70 cm
produced the longest roots but were not significantly different with the root lengths
obtained from banana plantlets grown in burnt rice hull only with a mean of 27.40 cm.
Furthermore plantlets grown in Enrico Organic Growing Media alone produced the shortest
roots,45 days from transplanting from the culture bottle.
As previously mentioned the results collaborated with the earlier findings of
Gawadan (1999) who found that a mixture of 1:1:1 alnus compost + rice hull + river sand
had significantly promoted longer roots in banana plantlets.
Table 3. Root length of banana plantlets after 45 days from planting
TREATMENT
MEAN
(cm)
Farmers practice (garden soil and alnus compost)
16.77cde
Alnus Compost
15.07de
Sandy Soil
22.80bc
Burnt rice hull
27.40ab
Fern dust
30.70a
Enrico Organic Growing Media
13.00e
AC + S + BRH + FD +Enrico Organic Growing Media
19.70cd
CV (%)
16.81%
Means with common letter are not significantly different at 5% level of DMRT
Acclimatization of Tissue Cultured Banana Plantlets (var. ‘Lakatan’) as Affected by
Different Growing Media | PEG-ED, JIMUEL P. OCTOBER 2012
Number of Leaves
Table 4. Banana plantlets grown in fern dust only significantly produced more
leaves with a mean of 5.33 after 45 days from transplanting from the cultured bottle; but
were not significantly different from plantlets grown in Enrico Organic Growing Media
only and alnus compost + mountain sand + burnt rice hull + fern dust +enrico growing
mediawhich had a mean of 4.00.
On the other hand, Acop (1987) as cited by Daculan (2010) reported that a medium
of 1:1:1 part of horse manure + garden soil + alnus compost produced the tallest plants
with the highest leaf count at anthesis and promoted earlier of initiation flowers in
chrysanthemum plantlets grown from in vitro.
Table 4. Number of leaves per plant of banana plantlets after 45 days from planting
TREATMENT
MEAN
Farmers practice (garden soil and alnus compost)
3.00 b
Alnus Compost
3.00 b
Sandy Soil
3.00 b
Burnt rice hull
3.00 b
Fern dust
5.33a
Enrico Organic Growing Media
4.00ab
AC + S + BRH + FD +Enrico Organic Growing Media
4.00ab
CV (%)
22.75%
Means with common letter are not significantly different at 5% level of DMRT
Acclimatization of Tissue Cultured Banana Plantlets (var. ‘Lakatan’) as Affected by
Different Growing Media | PEG-ED, JIMUEL P. OCTOBER 2012
Percentage Survival
Banana plantlets grown in the different growing media did not show significant
differences in percentage survival obtained 45 days after transplanting from the culture
bottle as shown in Table 5.
Table 5. Percentage survival of banana plantlets after 45 days from planting
TREATMENT
MEAN
Farmers practice (garden soil and alnus compost)
100
Alnus Compost
100
Sandy Soil
100
Burnt rice hull
100
Fern dust
100
Enrico Organic Growing Media
100
AC + S + BRH + FD +Enrico Organic Growing Media
100
CV (%)
00.00%
Means with common letter are not significantly different at 5% level of DMRT
Cost and Return Analysis
Table 6 shows that higher return on investment was obtained with the use of sand
only followed by usingburnt rice hull only and the farmers practice and alnus compost only.
Acclimatization of Tissue Cultured Banana Plantlets (var. ‘Lakatan’) as Affected by
Different Growing Media | PEG-ED, JIMUEL P. OCTOBER 2012
Table 6. Cost and return analysis of banana plantlets
TREATMENT_________________
T1
T2 T3 T4 T5
T6
T7
Plantlets that survived (pcs.) 24
24 24 24 24
24
24
Gross income
480 480 480 480 480 480 480
Expenses
Plantlets (P)
179 179 179 179 179 179 179
Growing media 30 30 10 20 50 75 100
Fungicide
20 20 20 20 20 20 20
Seedling box 30 30 30 30 30 30 30
Labor
20
20 20 20 20
20
20
Total expenses
279 279 259 269 299 324 349
Net income (P)
201
201 221 211 181 156 131
ROI (%)
72.04 72.04 82.16 78.44 60.53 48.15 37.54
Rank
3
3
1 2 4 5
6
Total sales per treatment were based at wholesale price of P 20.00 per piece.
Legend:
T1
Farmers Practice
T2
Alnus Compost
T3
Sandy Soil
T4
Burnt Rice Hull
T5
Fern Dust
T6
Enrico Organic Growing Media
T7
AC + MS + BRH + FD + Enrico Organic
Growing Media (1:1:1:1:1)
Acclimatization of Tissue Cultured Banana Plantlets (var. ‘Lakatan’) as Affected by
Different Growing Media | PEG-ED, JIMUEL P. OCTOBER 2012
Pictorial Presentation
Figure 1. Overview of the experimental area three weeks (21 days) after transplanting
from the culture bottle
.
Acclimatization of Tissue Cultured Banana Plantlets (var. ‘Lakatan’) as Affected by
Different Growing Media | PEG-ED, JIMUEL P. OCTOBER 2012
2
A
B
C
D
E
F
G
Figure 2. Banana plantlets grown in different growing media 21 days after transplanting
from the culture bottle A-alnus compost+garden soil; B-alnus compost; C-sand;
D-burnt rice hull; E-fern dust; F-commercial fertilizer; G- alnus compost+ sand+
burnt rice hull+ fern dust+ enrico organic growing media
Acclimatization of Tissue Cultured Banana Plantlets (var. ‘Lakatan’) as Affected by
Different Growing Media | PEG-ED, JIMUEL P. OCTOBER 2012
SUMMARY, CONCLUSION AND RECOMMENDATION
Summary
The study was conducted at La Trinidad Benguet from March to May 2012 to
establish the best growing media to harden the rooted banana plantlets from in vitro before
transplanting them in the field; and to evaluate the growth of banana plantlets from in vitro
grown in different growing media and media compositions.
A medium of fern dust only and alnus compost + sand+ burnt rice hull+ fern dust+
Enrico Organic Growing Mediasignificantly promoted the growth of more vigorous
plantlets and enhanced faster growth and development of banana plantlets grown in vitro.
Moreover plantlets grown in fern dust only produced the longest roots and higher number
leaves per plant. Furthermore banana plantlets grown in different growing media had no
significant differences in terms of percentage survival, 45 days after transplanting from the
culture bottles.
However, the highest return on investment was obtained with then use of sandy soil
only followed by burnt rice hull only as growing medium.
Conclusion
Based on the results of the study, fern dust only and alnus compost + sand+ burnt
rice hull+ fern dust+ Enrico Organic Growing Mediawere the best media in acclimatizing
banana plantlets.
Acclimatization of Tissue Cultured Banana Plantlets (var. ‘Lakatan’) as Affected by
Different Growing Media | PEG-ED, JIMUEL P. OCTOBER 2012
Recommendation
From the preceding results, acclimatizing banana plantlets in fern dust only for
economic reasons and or in a composition of alnus compost + sand+ burnt rice hull+ fern
dust+Enrico Organic Growing Mediais therefore recommended. It is further recommended
that in acclimatizing banana plantlets the following steps should be followed:
a.) Remove rooted tissue cultured plantlets from the culture bottle and wash with
tap water to remove the gelling agent;
b.) Soak plantlets in Previcure fungicide solution (2ml/li) for five minutes then
were transplant in fern dust growing media;and,
c.) Before transplanting, the growing media should be drenched with Previcure
fungicide (2ml/li) to avoid unnecessary infection.
Acclimatization of Tissue Cultured Banana Plantlets (var. ‘Lakatan’) as Affected by
Different Growing Media | PEG-ED, JIMUEL P. OCTOBER 2012
LITERATURE CITED
ALADOG, N.K. 2005. Effect of Different Potting Media on The Growth and flowering
of Zenia (Zenie elegance). BS Thesis. Benguet State University, La Trinidad,
Benguet.
ANONYMOUS. The Australia and Pacific Science Foundation. Accessed on July 17, 2012
from http://www.en.wikipedia.org/wiki/Banana.
AZCON-AGUILAR,C.,M. CANTOS, A. TRANCOSO, and J.M BAREA. 1997.
Beneficial Effect of Arbuscular Mycorrhizas on Acclimatization of
Micropropagated Cassava Plantlets. Scientia Hort. 72:63-71.
BAUTISTA, O.K and R.C. MABESA. 1997. Cabbage Production. College of Agriculture,
University of the Philippines, Los Banos. Laguna Pp. 45-46
CABALO, C.T. 2001. Growth And Flowering of Milflowers (Hydrangea macro phyla) as
Affected by Different Potting Media Compositions. BS Thesis. Benguet State
University, La Trinidad, Benguet. Pp 17-21.
CARVALHO,L.C., M.L. OSARIO, M.M. CHAVES, and S. AMANCIO. 2001.
Chlorophyll Fluorescence as Indicator of Photosynthetic Functioning Of In Vitro
Grapevine and Chestnut Plantlets under Ex Vitro Acclimatization. Plant Cell Tissue
Culture and Organ Culture. Pp 32-35.
CASSELLS, A. C. and C. WALSH. 1994. The Influence of Gas Permeability ofthe Culture
Lid on Calcium Uptake and Stomatal Function in Dianthus Microplants. Plant Cell
Tissue Organ Culture 37:171-178.
CRUCIDO, S.S., E.D.A VEDA, T. F TEDORA, R.H. DILOY, D.H MOJICA, and A.B.
MOLINA. 2007. Rehabilitation of Banana in Cavite through the use of local and
introduced banana cultivars propagated by tissue culture. Accessed on July 24,2012
from http://www.Musalit.org.
DE FOSSARD, R. A. 1981. Tissue Culture Micropopagation, Notes on Tissue Culture.
Enfield, N. H. Science Publishers, Inc. Pp 34-37.
DUMASLAN, M R. 2006. Growth Performance of Hardened Tissue Cultured Strawberry
Plantlets Under Greenhouse Condition.MS Thesis, BSU La Trinidad, Benguet. P
7.
ISAA, 2006.Tissue Culture Technology. International Service for the Acquisition of Agri-
biotic
Applications.
(ISAA).
Accessed
on
July
4,2012
from
http//www.isaa.org/resources.aspx.
Acclimatization of Tissue Cultured Banana Plantlets (var. ‘Lakatan’) as Affected by
Different Growing Media | PEG-ED, JIMUEL P. OCTOBER 2012
JANNA, O. A., R.OHIDIN, and M. MAZIAH. 2005. Glass House Acclimatization of
Tissue-Cultured Melastoma Malabathricum Plantlets. Tropical Sci. 45(1):45-49.
MORGAN, Ed R., J. H., and G. K. BURGE. 2003. Acclimatizing Tissue Culture Plants:
Reducing the Shock. New Zealand Institute for Crop & Food Res. Lt, Prvt Bag 11
600, Palmerston North, New Zealand. Pp 22-26.
PCARRD.1992 and 1988. The Philippines Recommends for Banana. Philippine
Agriculture and Resources Research Foundation Inc. Los Banos, Laguna. Pp. 1, 11-
20.
RAZDAN, M. K. 1996. Introduction to Plant Tissue Culture (2nd edition). Enfield, N. H.
Science Publishers, Inc. Pp 375.
RECEL, M. R. 2004. Banana Production Manual. Horticulture Information (HORTINET),
PCARRD-DOST. Los Banos, Laguna. Pp. 1, 3-19, 32.
SHARMA, S. D. 1998. Endogenous plant growth regulators and rooting capacity of on
their survival during acclimatization. Tropical Sci. 45(1): 45-49.
SLUIS, C. J. 2005. Protocols Greenhouse Planting of Tissue Culture Transplants. In Plant
Tissue Culture Engineering (Gupta, S. D. and Ibaraki,Y.Eds).Springer, 2006. Pp
231-251.
VALMAYOR, R. V. International Network for Improvement of Banana and Plantain. Asia
Pacific
Office.Accessed
on
July
17,
2012
from
http://www.wikwipedia.org/wiki/Banana.
Acclimatization of Tissue Cultured Banana Plantlets (var. ‘Lakatan’) as Affected by
Different Growing Media | PEG-ED, JIMUEL P. OCTOBER 2012
PEG-ED, JIMUEL P. OCTOBER 2012. Acclimatization of Tissue Cultured
Banana Plantlets (var. ‘Lakatan’) as Affected by Different Growing Media. Benguet State
University, La Trinidad, Benguet.
Adviser: Leila Mary A. Ayban, MSc.
ABSTRACT
The study was conducted at the Ornamental Horticulture Area, Benguet State
University, La Trinidad, Benguet from March 2012 to May 2012. The study was conducted
to establish the best growing media to harden the rooted banana plantlets from in vitro
before transplanting them to the field; and to evaluate the growth of banana plantlets from
in vitro grown in different growing media and media compositions.
Results revealed that a medium of fern dust alone; and alnus compost + burnt rice
hull+ fern dust+ Enrico Organic Growing media significantly promoted more vigorous
plantlets and enhanced better growth and development of banana plantlets. Moreover
plantlets grown in fern dust produced the longest roots and higher number of leaves per
plant.
Furthermore, banana plantlets grown in the different growing media had no
significant differences in terms of percentage survival, which was recorded 45 days after
transplanting from the culture bottles.
In addition, fern dust alone; and alnus compost + burnt rice hull+ fern dust+ Enrico
Organic growing media were found to be the best growing media in acclimatizing banana
plantlets from in vitro.
Acclimatization of Tissue Cultured Banana Plantlets (var. ‘Lakatan’) as Affected by
Different Growing Media | PEG-ED, JIMUEL P. OCTOBER 2012
RESULTS AND DISCUSSIONS
Plant Vigor
Table 1 shows the plant vigor of banana plantlets grown in different growing media
after 45 days. Plantlets grown in sandy soil were significantly more vigorous with a mean
rating of 2.33 but were not significantly different to those plantlets grown in garden soil +
alnus compost (farmers practice), and inalnus compost only, burnt rice hull only, and
Enrico Organic growing media. Moreover, plantlets grown in fern dust only and alnus
compost + sand + burnt rice hull + fern dust + Enrico Organic Growing Media were the
least vigorous.
Table 1. Plant vigor rating index of banana plantlets after 45 days from planting
TREATMENT
MEAN
Farmers practice (garden soil and alnus compost)
2.00a
Alnus Compost
2.00a
Sandy Soil
2.33a
Burnt rice hull
2.00a
Fern dust
1.00b
Enrico Organic Growing Media
2.00a
AC + S + BRH + FD +Enrico Organic Growing Media
1.00b
CV (%)
12.39
%
Means with common letter are not significantly different at 5% level of DMRT
Acclimatization of Tissue Cultured Banana Plantlets (var. ‘Lakatan’) as Affected by
Different Growing Media | PEG-ED, JIMUEL P. OCTOBER 2012
Plant Height
As presented in Table 2 banana plantlets grown in alnus compost + sand + burnt
rice hull + fern dust + Enrico Organic Growing Media had significantly faster plant growth
which was not significantly different with those of the plantlets grown in fern dust onlyas
compared to the other growing media.
The presence of sand and burnt rice hull in the alnus compost media improved the
physical properties of the medium resulting to better drainage and aeration leading to better
plant growth performance (Dumaslan, M. 2006).
Table 2. Plant height of banana plantlets after 45 days from planting
TREATMENT
MEAN
(cm)
Farmers practice (garden soil and alnus compost)
12.56b
Alnus Compost
12.33 b
Sandy Soil
12.60 b
Burnt rice hull
14.03 b
Fern dust
26.33a
Enrico Organic Growing Media
12.57 b
AC + S + BRH + FD +Enrico Organic Growing Media
28.23a
CV (%)
11.65%
Means with common letter are not significantly different at 5% level of DMRT
Acclimatization of Tissue Cultured Banana Plantlets (var. ‘Lakatan’) as Affected by
Different Growing Media | PEG-ED, JIMUEL P. OCTOBER 2012
Root Length
Table 3.Shows that significant or differences were observed among the different
growing media used. Banana plantlets grown in fern dust only with a mean of 30.70 cm
produced the longest roots but were not significantly different with the root lengths
obtained from banana plantlets grown in burnt rice hull only with a mean of 27.40 cm.
Furthermore plantlets grown in Enrico Organic Growing Media alone produced the shortest
roots,45 days from transplanting from the culture bottle.
As previously mentioned the results collaborated with the earlier findings of
Gawadan (1999) who found that a mixture of 1:1:1 alnus compost + rice hull + river sand
had significantly promoted longer roots in banana plantlets.
Table 3. Root length of banana plantlets after 45 days from planting
TREATMENT
MEAN
(cm)
Farmers practice (garden soil and alnus compost)
16.77cde
Alnus Compost
15.07de
Sandy Soil
22.80bc
Burnt rice hull
27.40ab
Fern dust
30.70a
Enrico Organic Growing Media
13.00e
AC + S + BRH + FD +Enrico Organic Growing Media
19.70cd
CV (%)
16.81%
Means with common letter are not significantly different at 5% level of DMRT
Acclimatization of Tissue Cultured Banana Plantlets (var. ‘Lakatan’) as Affected by
Different Growing Media | PEG-ED, JIMUEL P. OCTOBER 2012
Number of Leaves
Table 4. Banana plantlets grown in fern dust only significantly produced more
leaves with a mean of 5.33 after 45 days from transplanting from the cultured bottle; but
were not significantly different from plantlets grown in Enrico Organic Growing Media
only and alnus compost + mountain sand + burnt rice hull + fern dust +enrico growing
mediawhich had a mean of 4.00.
On the other hand, Acop (1987) as cited by Daculan (2010) reported that a medium
of 1:1:1 part of horse manure + garden soil + alnus compost produced the tallest plants
with the highest leaf count at anthesis and promoted earlier of initiation flowers in
chrysanthemum plantlets grown from in vitro.
Table 4. Number of leaves per plant of banana plantlets after 45 days from planting
TREATMENT
MEAN
Farmers practice (garden soil and alnus compost)
3.00 b
Alnus Compost
3.00 b
Sandy Soil
3.00 b
Burnt rice hull
3.00 b
Fern dust
5.33a
Enrico Organic Growing Media
4.00ab
AC + S + BRH + FD +Enrico Organic Growing Media
4.00ab
CV (%)
22.75%
Means with common letter are not significantly different at 5% level of DMRT
Acclimatization of Tissue Cultured Banana Plantlets (var. ‘Lakatan’) as Affected by
Different Growing Media | PEG-ED, JIMUEL P. OCTOBER 2012
Percentage Survival
Banana plantlets grown in the different growing media did not show significant
differences in percentage survival obtained 45 days after transplanting from the culture
bottle as shown in Table 5.
Table 5. Percentage survival of banana plantlets after 45 days from planting
TREATMENT
MEAN
Farmers practice (garden soil and alnus compost)
100
Alnus Compost
100
Sandy Soil
100
Burnt rice hull
100
Fern dust
100
Enrico Organic Growing Media
100
AC + S + BRH + FD +Enrico Organic Growing Media
100
CV (%)
00.00%
Means with common letter are not significantly different at 5% level of DMRT
Cost and Return Analysis
Table 6 shows that higher return on investment was obtained with the use of sand
only followed by usingburnt rice hull only and the farmers practice and alnus compost only.
Acclimatization of Tissue Cultured Banana Plantlets (var. ‘Lakatan’) as Affected by
Different Growing Media | PEG-ED, JIMUEL P. OCTOBER 2012
Table 6. Cost and return analysis of banana plantlets
TREATMENT_________________
T1
T2 T3 T4 T5
T6
T7
Plantlets that survived (pcs.) 24
24 24 24 24
24
24
Gross income
480 480 480 480 480 480 480
Expenses
Plantlets (P)
179 179 179 179 179 179 179
Growing media 30 30 10 20 50 75 100
Fungicide
20 20 20 20 20 20 20
Seedling box 30 30 30 30 30 30 30
Labor
20
20 20 20 20
20
20
Total expenses
279 279 259 269 299 324 349
Net income (P)
201
201 221 211 181 156 131
ROI (%)
72.04 72.04 82.16 78.44 60.53 48.15 37.54
Rank
3
3
1 2 4 5
6
Total sales per treatment were based at wholesale price of P 20.00 per piece.
Legend:
T1
Farmers Practice
T2
Alnus Compost
T3
Sandy Soil
T4
Burnt Rice Hull
T5
Fern Dust
T6
Enrico Organic Growing Media
T7
AC + MS + BRH + FD + Enrico Organic
Growing Media (1:1:1:1:1)
Acclimatization of Tissue Cultured Banana Plantlets (var. ‘Lakatan’) as Affected by
Different Growing Media | PEG-ED, JIMUEL P. OCTOBER 2012
Pictorial Presentation
Figure 1. Overview of the experimental area three weeks (21 days) after transplanting
from the culture bottle
.
Acclimatization of Tissue Cultured Banana Plantlets (var. ‘Lakatan’) as Affected by
Different Growing Media | PEG-ED, JIMUEL P. OCTOBER 2012
2
A
B
C
D
E
F
G
Figure 2. Banana plantlets grown in different growing media 21 days after transplanting
from the culture bottle A-alnus compost+garden soil; B-alnus compost; C-sand;
D-burnt rice hull; E-fern dust; F-commercial fertilizer; G- alnus compost+ sand+
burnt rice hull+ fern dust+ enrico organic growing media
Acclimatization of Tissue Cultured Banana Plantlets (var. ‘Lakatan’) as Affected by
Different Growing Media | PEG-ED, JIMUEL P. OCTOBER 2012
SUMMARY, CONCLUSION AND RECOMMENDATION
Summary
The study was conducted at La Trinidad Benguet from March to May 2012 to
establish the best growing media to harden the rooted banana plantlets from in vitro before
transplanting them in the field; and to evaluate the growth of banana plantlets from in vitro
grown in different growing media and media compositions.
A medium of fern dust only and alnus compost + sand+ burnt rice hull+ fern dust+
Enrico Organic Growing Mediasignificantly promoted the growth of more vigorous
plantlets and enhanced faster growth and development of banana plantlets grown in vitro.
Moreover plantlets grown in fern dust only produced the longest roots and higher number
leaves per plant. Furthermore banana plantlets grown in different growing media had no
significant differences in terms of percentage survival, 45 days after transplanting from the
culture bottles.
However, the highest return on investment was obtained with then use of sandy soil
only followed by burnt rice hull only as growing medium.
Conclusion
Based on the results of the study, fern dust only and alnus compost + sand+ burnt
rice hull+ fern dust+ Enrico Organic Growing Mediawere the best media in acclimatizing
banana plantlets.
Acclimatization of Tissue Cultured Banana Plantlets (var. ‘Lakatan’) as Affected by
Different Growing Media | PEG-ED, JIMUEL P. OCTOBER 2012
Recommendation
From the preceding results, acclimatizing banana plantlets in fern dust only for
economic reasons and or in a composition of alnus compost + sand+ burnt rice hull+ fern
dust+Enrico Organic Growing Mediais therefore recommended. It is further recommended
that in acclimatizing banana plantlets the following steps should be followed:
a.) Remove rooted tissue cultured plantlets from the culture bottle and wash with
tap water to remove the gelling agent;
b.) Soak plantlets in Previcure fungicide solution (2ml/li) for five minutes then
were transplant in fern dust growing media;and,
c.) Before transplanting, the growing media should be drenched with Previcure
fungicide (2ml/li) to avoid unnecessary infection.
Acclimatization of Tissue Cultured Banana Plantlets (var. ‘Lakatan’) as Affected by
Different Growing Media | PEG-ED, JIMUEL P. OCTOBER 2012
LITERATURE CITED
ALADOG, N.K. 2005. Effect of Different Potting Media on The Growth and flowering
of Zenia (Zenie elegance). BS Thesis. Benguet State University, La Trinidad,
Benguet.
ANONYMOUS. The Australia and Pacific Science Foundation. Accessed on July 17, 2012
from http://www.en.wikipedia.org/wiki/Banana.
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